Aeration is one of the important factors to produce monoclonal


Aeration is one of the important factors to produce monoclonal antibodies from Chinese hamster ovary (CHO) cell lines. Compressed air is continuously supplied for 2 weeks with a flow rate of 0.05 m3/min and velocity of 0.31 m/sec into a 2.5 L medium. It is found that the air provided contain 2.8 x 10 5 CFU/m3 of contaminants. Determine the dimension of the filter used, if the X90 value for cotton wool is 1.5 cm.

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